Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling SystemMichael K. Conway 1, Michael J. Gerger 1, Erin E. Balay 1, Rachel O'Connell 1, Seth Hanson 2, Neil J. Daily 1, Tetsuro Wakatsuki 1
1InvivoSciences, Inc., 2Gilson, Inc.
The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.
